Ionizing Radiation-Induced Extracellular Vesicle Release Promotes AKT-Associated Survival Response in SH-SY5Y Neuroblastoma Cells

Ionizing Radiation-Induced Extracellular Vesicle Release Promotes AKT-Associated Survival Response in SH-SY5Y Neuroblastoma Cells

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Radiation therapy is one of the most effective methods of tumor eradication; however, in some forms of neuroblastoma, radiation can increase the risk of secondary neoplasms, 14765-prb-a01 due to the ability of irradiated cells to transmit pro-survival signals to non-irradiated cells through vesicle secretion.The aims of this study were to characterize the vesicles released by the human neuroblastoma cell line SH-SY5Y following X-ray radiations and their ability to increase invasiveness in non-irradiated SH-SY5Y cells.We first purified the extracellular vesicles released by the SH-SY5Y cells following X-rays, and then determined their total amount, dimensions, membrane protein composition, and cellular uptake.We also examined the effects of these extracellular vesicles on viability, migration, and DNA damage in recipient SH-SY5Y cells.We found that exposure to X-rays increased the release of extracellular vesicles and altered their protein composition.

These vesicles were readily uptaken by non-irradiated cells, inducing an increase in viability, migration, and radio-resistance.The same results were obtained in purple ipad pro 12.9 case an MYCN-amplified SK-N-BE cell line.Our study demonstrates that vesicles released from irradiated neuroblastoma cells stimulate proliferation and invasiveness that correlate with the epithelial to mesenchymal transition in non-irradiated cells.Moreover, our results suggest that, at least in neuroblastomas, targeting the extracellular vesicles may represent a novel therapeutic approach to counteract the side effects associated with radiotherapy.